Production of polyhydroxyalkanoate from nipa sap using Cupriavidus necator DSM545

Abstract Polyhydroxyalkanoate or PHA is produced through microbial fermentation of sugar and lipid where in nature it stored as energy carbon source for microorganisms. Most the feedstock used production are derived from biomass they remain cheapest to date. In this study, nipa palm sap was proposed an alternative since underutilized widely distributed along river estuaries Malaysia. This contains high content essential nutrients which required production. The aim work explore potential a investigate effect added non-added nutrient media on during batch using Cupriavidus necator DSM545. Extraction chloroform showed that 2 g/L recovered when with while only 2.16 PHA. All samples presence carbonyl band (C=O) extracted granules by FTIR peak obtained UV Spectrophotometer confirmed These findings proved novel